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Cuyamaca College The Effect of Temperature on Lactase Activity Lab Report

Cuyamaca College The Effect of Temperature on Lactase Activity Lab Report

Question Description

I’m working on a biology report and need a sample draft to help me learn.

Enzyme Lab Write Up: You will submit this write up and graphs before 11:59 PM Sunday (Week 2). You will be provided with comments and peer feedback. For next week you may make changes based on these comments and feedback (or inspirations which come to you outside of this exercise) and submit a lab report prior to 11:59 PM next Sunday (Week 3). Lab for the Week 3 will consist of providing comments and feedback to your peers and reworking your lab report. We will not be doing the osmosis lab that week.

1. Produce a Lab Summary of the Effect of Temperature on Lactase Activity. I am providing you an outline, but your report should be in nonnumeric, paragraph form. Your writeup should be in a formal style. Your writeup should be clearly and formally written. Your writeup should have clearly identified sections (include headers for each section).

2. Write an Introduction Section (1 or 2 paragraphs) State the purpose of the Effect of Temperature on Lactase Activity experiment. Write a hypothesis for the experiment. Identify the independent variable and dependent variable for the experiment.

3. Background Research: Research your experiment. Write 2-3 paragraphs which give an overview of enzymes. Write a paragraph explaining lactase. Write a paragraph which states the expected results.

4. Graph your data. Remember the x-axis should show the independent variable (in this example, temperature) and the y-axis should show glucose produced. You should produce four (4) graphs: 1) a graph showing how you determined the inflection point of temperature compared to glucose produced with lines for 4 minutes, 7 minutes, and 11 minutes; 2) a graph showing the optimum temperature for glucose produced in 4 minutes; 3) a graph showing the optimum temperature for glucose produced in 7 minutes and; 4) a graph showing the optimum temperature for glucose produced in 11 minutes. You need to include a title for each graph. Use the following format: Figure Number: Title. Your title for each graph should be a sentence letting the reader know what the graph will tell them. (For example: Figure 2: Glucose production by lactase in 4 minutes between 5-20 0C , pH 7.0, initial lactose concentration 500 mg/dL )

5. Analyze the temperature effect on lactase activity data. (at least 3 paragraphs) This as an opportunity to tie together any relevant information you brought into the Introduction section and the results (data). Some of the information you report in the introduction should prove useful as you try to explain your results. Refer back to the graph (For example: See Figure 7) when you point out important ranges or specific temperatures of importance

6. If your actual experimental results did not match your expected results, write a paragraph suggesting one reason your actual results might have been different from the expected results.



Experiment II: The effect of temperature on lactase activity. Use the Experiment I to design an experiment to test the effect of temperature on lactase activity. What is the independent variable of the temperature experiment? What is the dependent variable of the temperature experiment? Which variables will you standardize in the temperature experiment?

I. Describe the experiment you designed to determine the inflection point and interval of interest for temperature.

II. Describe the experiment you designed to determine the optimum temperature. Use the tables to record and four graphs to report your data. What is the optimum temperature for lactose?

I. Determine the inflection point and interval of interest for temperature.

Temperature (C) 4 minutes 7 minutes 11 minutes




After you complete the table, use the data to produce a graph with all three times (4, 7, and 11 minutes) on the same graph. What is the interval of interest and inflection point based on your results? The interval of interest is the range of temperatures which will give you the most valuable, relevant data for determining the optimum temperature in the second part of the experiment.

II.Use the inflection and temperature interval of interest to design the second part of the experiment. Divide this interval into ten (10) equally spaced intervals. Use the table below to record the results of these simulations at 4 minutes, 7 minutes and 11 minutes for each temperature. Run the simulation and record the data.

Temperature (C) 4 minutes 7 minutes 11 minutes




Use the data from the table to produce three individual graphs: 1) temperature : glucose concentration at 4 minutes. 2) temperature : glucose concentration at 7 minutes, and 3) temperature : glucose concentration at 11 minutes. Look at the graphs and determine the optimum temperature (where maximum lactase enzyme activity is likely located).


All the graphs and tables need to be done in Excel, thank you for your time! (Both parts need to be done, the top and bottom parts).

(The temperature where it says 4/7/11 minutes are supposed to be tables but studypool didnt let me paste the tables on there so I left big blanks).


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